Nucleobase transport in cultured renal epithelial cells.

نویسندگان

  • R Akerman
  • S M Jarvis
چکیده

The translocation o f the purine nucleobases, guanine and hypoxanthine, across the plasma membrane of mammalian cells is the essential first step of the purine salvage pathway. Until recently, it was believed that such purines entered a variety o f cells via facilitated-diffusion systems that were either specific for nucleobases or also accepted nucleosides as permeants [ I , 21. However, this laboratory recentlydemonstrated the presence o f a sodium linked cotransporter for certain nucleobases in LLC-PK, cells, a cell line derived from the late proximal tubule o f the juvenile pig kidney [3]. To investigate h o w widespread this cotransporter is, w e have started t o study the properties of nucleobase transport in other epithelial cell lines, such as OK which is derived from the late proximal tubule o f the opossum kidney [41. In this report, w e describe the characterization of nucleobase transporters in the OK cells with particular emphasis on the permeant specificity of the carrier systems.

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عنوان ژورنال:
  • Biochemical Society transactions

دوره 23 1  شماره 

صفحات  -

تاریخ انتشار 1995